T. Wei1*, M.N. Pearson1 and D. Cohen2
1 School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland Mail Centre, Auckland 1142, New Zealand
2 Hortresearch, Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand
Accepted: 03 Oct 2006
Iris (Iridaceae) is a prized ornamental, valued for its beauty and as a source of perfumes and medicinal products. In New Zealand, irises are grown mostly for the domestic cut flower market, estimated at NZ$70 million in 2005 (Anon, 2005).
Iris tingitana plants showing leaf mosaic symptoms were collected from a commercial grower near Palmerston North, New Zealand in July 2004. Leaf samples with symptoms tested positive for potyvirus infection by ELISA, using a potyvirus universal monoclonal antibody (Agdia Inc., Elkhart, USA). Universal potyvirus primers PV2I/T7 and PV1/SP6 (Mackenzie et al., 1998) were used to amplify a product of approximately 1.8 Kb, spanning part of the NIb gene, the entire CP gene and the 3' UTR. The amplicon was cloned into the vector pGEM®-T Easy (Promega Corporation, Madison, WI, USA) and the sequence analyzed using BioEdit (version7.0.5) and DNA-star (version 4.0). The 1815 bp sequence (Genbank Accession No. DQ450199) showed 96% identity for both nucleotide and amino acid sequences to the only other example of Narcissus latent virus (NLV) listed in GenBank (Accession No. U58770; Badge et al. 1997). This is the first report of NLV in New Zealand.
Anonymous, 2005. Fresh facts: New Zealand Horticulture 2005. [http://www.hortresearch.co.nz/files/aboutus/factsandfigs/ff2005.pdf]
Badge J, Robinson DJ, Brunt AA, Foster GD, 1997. 3′ - Terminal sequences of the RNA genomes of narcissus latent and Maclura mosaic viruses suggest that they represent a new genus of the Potyviridae. Journal of General Virology 78, 253-257.
Mackenzie AM, Nolan M, Wei K-J, Clements MA, Gowanlock D, Wallace BJ, Gibbs AJ, 1998. Ceratobium mosaic potyvirus: another virus from orchids. Archives of Virology 143, 903-914.
©2006 The Authors