New Disease Reports (2013) 27, 20. [http://dx.doi.org/10.5197/j.2044-0588.2013.027.020]
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Phytophthora multivora causing leaf spot on rhododendrons in Argentina

P.E. Grijalba 1*, H.E. Palmucci 1, E. Guillin 2 and C. Herrera 1

*grijalba@agro.uba.ar

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Received: 19 Nov 2012; Published: 18 Jun 2013

Keywords: azalea, oomycete, plant disease

Rhododendron is a genus belonging to the family Ericaceae and contains over 1000 species commonly known as rhododendrons and azaleas (Dimitri, 1978). Phytophthora multivora is a species that was described in Western Australia in 2008 but before that it was misidentified as P. citricola (Scott et al., 2009). During the early spring of 2011, leaf spot symptoms were observed on rhododendrons in two gardens in Tigre (northern Buenos Aires province) and in containers in a nursery near Buenos Aires city. Leaf spots were dark brown to almost black, visible on both sides near the leaf tips and margins, while tissue death continued down the leaf along the midrib (Fig. 1A). The veins under the diseased area of the leaves presented a reddish tint (Fig. 1B). Some leaves became brown and died, while in others the infection remained as spots on leaves.

A Phytophthora species was consistently isolated from symptom-bearing leaf tissues on PARBH medium (Jeffers & Martin, 1986). Hyphal tips reaching the surface of the medium were transferred onto potato dextrose agar (PDA) without inhibitors for purification and identification. Sporangia were produced abundantly in non-sterile soil extract. The majority of them were semi-papillate and ovoid, limoniform, ellipsoid or obpyriform (Fig. 2). Sporangia with two papillae were occasionally formed (Scott et al., 2009). Chlamydospores were not observed. Isolates were homothallic with plerotic oospores, 22.9 ± 1.9 μm and paragynous antheridia (Fig. 3). The optimum growth temperature was 25 ± 1°C on V8A (Scott et al., 2009) and the maximum growth temperature was 32 ± 1°C. The ITS was amplified and sequenced (GenBank Accession No. JQ812127) and showed that it was identical to P. multivora, ex-type CBS 124.094 (FJ237517) by BLAST analysis (Altschul et al., 1997). The isolate (ARod 110) was deposited in the culture collection of the Phytopathology Chair of the Faculty of Agronomy of Buenos Aires (FAUBA).

Due to the difficulty of performing pathogenicity tests in the field, detached leaves of Rhododendron spp., Camellia sp. Viburnum tinus and Photinia fraseri were inoculated in the laboratory with the ARod 110 isolate. The tests were performed by inoculating five detached leaves with a 5 mm mycelium plug taken from a seven-day-old PDA culture. Controls were inoculated with PDA discs. Leaves were incubated at 20-22ºC under 12h light/12h dark cycle. All the inoculated leaves, except Camellia sp., developed necrotic lesions seven days after inoculation. P. multivora was re-isolated from infected tissue. Symptoms were not detected on the controls. These characteristics conformed to those of Phytophthora multivora Scott & Jung. To our knowledge, this is the first record of P. multivora causing leaf spot on Rhododendron in Argentina and in Latin America, and it may be a potential pathogen for Viburnum tinus and Photinia fraseri.

Figure1+
Figure 1: Leaf spot symptoms of Phytophthora multivora on upper surface (A) and lower surface (B) of rhododendron leaves.
Figure 1: Leaf spot symptoms of Phytophthora multivora on upper surface (A) and lower surface (B) of rhododendron leaves.
Figure2+
Figure 2: Papillate sporangium of Phytophthora multivora. (Bar = 20 μm).
Figure 2: Papillate sporangium of Phytophthora multivora. (Bar = 20 μm).
Figure3+
Figure 3: Plerotic oospore and paragynous antheridium of Phytophthora multivora. (Bar = 10 μm).
Figure 3: Plerotic oospore and paragynous antheridium of Phytophthora multivora. (Bar = 10 μm).

Acknowledgements

The authors would like to thank Gloria Abad (USDA/ APHIS/ PPQ/ PHP/ RIPPS) for sharing her knowledge with us.


References

  1. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ, 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acid Research 25, 389-402. [http://dx.doi.org/10.1093/nar/25.17.3389]
  2. Dimitri MJ, 1978. Enciclopedia Argentina de Agricultura y Jardinería. Tomo I. Descripción de las plantas cultivadas. Segundo volumen. 3ª edición. Editorial ACME S.A.C.I., Buenos Aires, Argentina, 855-858.
  3. Jeffers SN, Martin SB, 1986. Comparison of two media selective for Phytophthora and Pythium species. Plant Disease 70, 1038-1043. [http://dx.doi.org/10.1094/PD-70-1038]
  4. Scott PM, Burgess TI, Barber PA, Shearer BL, Stukely MJC, Hardy GEStJ, Jung T, 2009. Phytophthora multivora sp. nov., a new species recovered from declining Eucalyptus, Banksia, Agonis and other plant species in Western Australia. Persoonia 22, 1-13. [http://dx.doi.org/10.3767/003158509X415450]

To cite this report: Grijalba PE, Palmucci HE, Guillin E, Herrera C, 2013. Phytophthora multivora causing leaf spot on rhododendrons in Argentina. New Disease Reports 27, 20. [http://dx.doi.org/10.5197/j.2044-0588.2013.027.020]

©2013 The Authors