New Disease Reports (2007) 15, 27.

First report of Beet soil-borne virus on sugar beet in China

Bin Wang, Min Li, Jingji Zhang, Chenggui Han*, Dawei Li and Jialin Yu

*Hanchenggui@cau.edu.cn

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Accepted: 27 Mar 2007

Beet soil-borne virus (BSBV) and Beet virus Q (BVQ) are two members of the genus Pomovirus that are transmitted by Polymyxa betae. BVQ and BSBV are commonly found in fields where Beet necrotic yellow vein virus (BNYVV), the causal agent of rhizomania disease, is present (Henry et al.1986; Koenig et al. 1998; Meunier et al. 2003). During the growing season of 2006, twenty six roots of sugar beet exhibiting moderate to severe symptoms of rhizomania were collected from three fields located in Inner Mongolia and Xinjiang Provinces for a survey to determine whether BSBV and BVQ were present in China. Viral RNA was extracted from partially purified viruses of beet roots as described by Han et al (2000) and tested by RT-PCR using three specific primer sets (BSBV: 5`- ATG GTT GAT CCG CGG TAT GAA G -3` and 5`- CTA TTC AAC CCA GCG CAA ACC AAC-3`; BVQ: 5`- ATG GTT GAT CCA AGA TAT GAG C -3` and 5`-CTA TGA GCC GGT CCA CTT CAA TCC-3`; and BNYVV: 5`- GCG GAT CCA TGT CGA GTG AA G GTA GA -3` and 5`-CGA AGC TTG CTA TTG TCC GGG TGG-3`, based on Acc. Nos. NC003518, NC003511 and S71490 respectively) and designed to amplify coat protein genes for BSBV, BVQ and BNYVV respectively.

Fifteen of the 26 beet samples gave positive reactions for BSBV, 21 for BNYVV and 14 for both BSBV and BNYVV (Fig. 1). BVQ was not detected in any sugar beet sample tested. Subsequently, three RT-PCR products amplified in sugar beet samples collected from different locations with the primers specific to BSBV were cloned and sequenced (GenBank Accession No. EF113300, EF210553 and EF210552). Sequence analysis revealed that these submitted sequences shared nucleotide identity of 98-99 % with the German BSBV isolate (Acc. No. NC003518). The partially purified viruses from infected sugar beet root were used for mechanical inoculation of Chenopodium quinoa and C. amaranticolor, and local lesions were observed on inoculated leaves (Fig. 2). Furthermore, BSBV was also confirmed by RT-PCR (as described above) in the local lesions that appeared approximately 5 days after mechanical inoculation.

Combined, the RT-PCR detection, nucleotide sequences and symptoms on inoculated host plants confirm the presence of BSBV in Inner Mongolia and Xinjiang. To our knowledge, this is the first report of the occurrence of BSBV in China.

Figure1+
Figure 1: Detection of BSBV (Top) and BNYVV (Bottom) by RT-PCR amplification of coat protein genes of 495bp and 567bp performed on sugar beet samples, respectively. Specific primer pairs for BSBV and BNYVV CP were used for amplification. Lanes 1-6, sugar beet samples collected from fields; Lane P, positive control; Lane H, healthy control; Lane M, λ DNA digested with EcoRI and HindIII.
Figure 1: Detection of BSBV (Top) and BNYVV (Bottom) by RT-PCR amplification of coat protein genes of 495bp and 567bp performed on sugar beet samples, respectively. Specific primer pairs for BSBV and BNYVV CP were used for amplification. Lanes 1-6, sugar beet samples collected from fields; Lane P, positive control; Lane H, healthy control; Lane M, λ DNA digested with EcoRI and HindIII.
Figure2+
Figure 2: Local lesions caused by Beet soil-borne virus on Chenopodium quinoa leaves appeared approximately 5 days after mechanically inoculation with extract from infected sugar beet root. The Brit
Figure 2: Local lesions caused by Beet soil-borne virus on Chenopodium quinoa leaves appeared approximately 5 days after mechanically inoculation with extract from infected sugar beet root. The Brit

Acknowledgements

This work was partially supported by the National Natural Science Foundation of China (30471136 and 30671359).


References

  1. Han CG, Li DW, Yu J L, Qin SC, Yang LL, Liu Y, 2000. Location of the deleted sequence of spontaneously deleted RNAs of Beet necrotic yellow vein virus NM isolate. Chinese Journal of Virology 16, 49-53.
  2. Henry CM, Jones RAC, 1986. Occurrence of a soil-borne virus of sugar beet in England. Plant Pathology 35, 585-591.
  3. Koenig R, Pleij CWA, Beier C, Commandeur U, 1998. Genome properties of Beet virus Q, a new furo-like virus from sugarbeet, determined from unpurified virus. Journal of General Virology 79, 2027-2036.
  4. Meunier A, Schmit JFO, Stas A, Kutluk N, Bragard C, 2003. Multiplex Reverse Transcription-PCR for Simultaneous Detection of Beet Necrotic Yellow Vein Virus, Beet Soilborne Virus and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet. Applied and Environmental Microbiology 69, 2356-2360.ish Society for Plant Pathology

This report was formally published in Plant Pathology

©2007 The Authors