First report of Tomato yellow leaf curl China virus infecting kidney bean in China
Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences, Kunming 650223, China
Accepted: 16 May 2006
In 2004, a virus isolate (Bean-YM) was obtained from Kidney bean (Phaseolus vulgaris) showing leaf curl symptoms (Fig. 1) from Yuanmou, in the Yunnan province of China. To identify possible begomoviruses, total DNA was extracted from a leaf sample. Degenerate primers PA and PB, designed to amplify part of the intergenic region and coat protein (CP) gene of DNA-A of begomoviruses (Zhou et al., 2003), were used in PCR. A 500 bp DNA fragment was obtained, cloned and sequenced. Sequence comparison showed that the fragment was most closely related to Tomato yellow leaf curl China virus (TYLCCNV) isolate [Y43] (Acc. No. AJ781302) with 92% nucleotide sequence identity. Primers WTGs-1 (5'-CGTGCT GCTGTCCCCACTGT-3') and WTGs-2 (5'-GTGGAAATGACT ATATCGGC-3') were then designed to amplify the remaining DNA-A sequences of Bean-YM. The complete DNA-A sequence of Bean-YM was determined to be 2731 nucleotides (DQ256460). Comparisons with other begomoviruses showed that DNA-A of Bean-YM is closely related to TYLCCNYV-[Y43] with 92% nucleotide sequence identity.
All previously characterised TYLCCNV isolates have been shown to be associated with DNA ÃŸ molecules (Cui et al., 2004). To test whether a satellite molecule is associated with this isolate, a universal abutting primer pair specific for DNA β was used to amplify the putative DNA ÃŸ (Briddon et al., 2002). Using this primer set, an amplicon of approximately 1400 bp was obtained. Sequence analysis revealed that DNA ÃŸ of Bean-YM is 1336 nucleotides long (DQ256459) and most closely related to the DNA β associated with TYLCCNV-[Y10] (AJ421621; 80.8% nucleotide sequence identity).
TYLCCNV has previously been reported to infect tomato, tobacco and weeds. This is the first report of TYLCCNV infecting a legume.
This work was supported by the National Natural Science Foundation of China( Grant No.30360005) and the Natural Science Foundation of Yunnan Province, China(Grant No. 2005C0012Z).
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This report was formally published in Plant Pathology
©2006 The Authors