Bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis, on tomatoes in Eastern Anatolia region of Turkey
1 Department of Plant Protection, Faculty of Agriculture, Atatürk University, 25240 Erzurum, Turkey
2 Biotechnology Research and Application Centre, Atatürk University, 25240 Erzurum, Turkey
Accepted: 22 Jan 2002
In 2001, a disease occurred on tomato (Lycopersicon esculentum Mill.) cv. Target growing in six different commercial fields in the production areas of Oltu, Ispir and Yusufeli in the Eastern Anatolia region of Turkey. Disease incidence was almost 100%, resulting in heavy losses. Initial symptoms were small (1-3 mm in diameter), water-soaked, dark brown to black lesions on the leaf margins and stems, and white, raised "bird's eye" spots on fruits. Defoliation, one-sided wilting, tip die-back, open stem cankers and vascular discoloration were observed on plants in advanced stages of the disease. These symptoms were consistent with those of bacterial canker described by Vidaver & Davis (1988) and were found only on cv. Target. A slow-growing, Gram-positive, pale-yellow bacterium was consistently isolated on Yeast Dextrose Carbonate Agar medium (Lelliot & Stead, 1987). Sixteen representative isolates were identified as Clavibacter michiganensis subsp. michiganensis (similarity 47 to 89 %) using fatty acid methyl ester (FAME) analysis and Sherlock Microbial Identification System software (Microbial ID, Newark, DE, USA) (Gitaitis & Beaver, 1990). Pathogenicity was tested by spraying 5-wk-old tomato seedlings (cv. Easy Harvest) with bacteria suspended (108 cfu/ml) in sterile water. Control plants were sprayed with sterile water. After inoculation plants were covered with polyethylene bags for 48 h and maintained in a greenhouse at 25°C. Symptoms similar to those observed in the field developed on the inoculated plants within 7-13 days. No symptoms developed on control plants. The bacterium was re-isolated from inoculated plants and its identity confirmed by FAME analysis. Although it has been reported previously in Aegean and Mediterranean regions (Ã–zaktan 1991), this is the first record of bacterial canker on tomatoes in the Eastern Anatolia region of Turkey. Occurrence of the disease only on plants of cv. Target suggests that contaminated seeds and/or transplants of cv. Target may have been the source of the pathogen.
- Gitaitis RD, Beaver RW, 1990. Characterization of fatty acid methyl ester content of Clavibacter michiganense subsp. michiganense. Phytopathology 80, 318-321.
- Lelliot RA, Stead DE, 1987. Methods for the Diagnosis of Bacterial Diseases of Plants. Oxford, UK: Blackwell Scientific Publications.
- Özaktan H, 1991. Domates Bakteriyel Solgunlugu (Clavibacter michiganensis subsp. michiganensis) ile Savaşım Olanakları Ãœzerine Araştırmalar. Izmir, Turkey: Ege University, PhD thesis.
- Vidaver AK, Davis MJ, 1988. Coryneform plant pathogens. In: Schaad, NW, ed. Laboratory Guide for Identification of Plant Pathogenic Bacteria, St. Paul, Minnesota, USA: The American Phytopathological Society, 60-80.
This report was formally published in Plant Pathology
©2002 The Authors