Shirin Farzadfar1*, Reza Pourrahim1, Ali Reza Golnaraghi2, Nooh Shahraeen1 and K.M. Makkouk3
1 Plant Virology Department, Plant Pests and Diseases Research Institute, P.O. Box 19395-1454, Tehran, Iran
2 Research & Science Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran
3 Virology Laboratory, Germplasm Program, ICARDA. P.O. Box 5466, Aleppo, Syria
Accepted: 08 Apr 2002
A survey was conducted during August 2001 to identify viruses infecting sugarbeet (Beta vulgaris) crops in the six provinces of Iran, including Semnan, Khorasan, Kermanshah, Qazvin, Fars and Esfahan. A total of 4795 leaf samples were collected randomly from 60 sugarbeet fields. Some samples showed chlorosis and stunting (Figure 1). In addition, two bean (Phaseolus vulgaris) fields with severe stunting, chlorosis and poor pod setting (Figure 2) were observed near sugar beet fields in Kermanshah, and 125 samples were collected from this location.
These samples were tested for the presence of Chickpea chlorotic dwarf virus (CpCDV, genus Mastrevirus, family Geminiviridae) by tissue blot immunoassay (TBIA) (Lin et al., 1990) using CpCDV-specific polyclonal antibody (provided by Dr. Vetten, DSMZ-Germany). The overall incidences of CpCDV in the sugar beet and bean fields surveyed were 15% and 67%, respectively. Immunosorbent electron microscopy showed Geminivirus particles in crude extracts of infected sugar beet and bean leaf samples. Crop losses in some bean fields in Kermanshah province were estimated at more than 60%. CpCDV has been previously reported in chickpea (Cicer arietinum) from Iran (Makkouk et al., 2001), but to our knowledge this is the first report of the natural occurrence incidence of CpCDV in sugarbeet and bean from Iran.
Lin NS, Hsu YH, Hsu HT, 1990. Immunological detection of plant viruses and a mycoplasma-like organism by direct tissue blotting on nitrocellulose membranes. Phytopathology 80, 824.
Makkouk KM, Fazlali Y, Kumari SG, Farzadfar Sh, 2002. First records of Beet western yellows virus, Chickpea chlorotic dwarf virus, Faba bean necrotic yellows virus and Soybean dwarf virus infecting chickpea and lentil crops in Iran. New Disease Reports [http://www.bspp.org.uk]. Volume 4.
©2002 The Authors