New Disease Reports (2017) 36, 9. [http://dx.doi.org/10.5197/j.2044-0588.2017.036.009]
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First report of Grapevine fanleaf virus infecting grapevine in the United Kingdom

G. Silva 1*, J. Lecourt 2, G.R.G. Clover 3 and S.E. Seal 1

*g.silva@gre.ac.uk

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Received: 15 Aug 2017; Published: 25 Sep 2017

Keywords: detection, GFLV, Vitis vinifera

The UK wine industry is a fast-growing sector and in 2015 an area of c. 2,000 hectares had been planted with vines from which over five million bottles of wine were produced (Wine and Spirit Trade Association Market Overview, 2016). It is important to monitor the phytosanitary status of vines to ensure the sustainability of the industry in the UK. 

In April 2017, four samples were collected. These were one dormant cane, selected at random, from each of four geographically separated vineyards which were from Pinot Noir clones (119, 336, 792 and 924) reciprocally grafted upon Gravesac, SO4 or 3309 Couderc rootstocks. RNA was extracted from cambial scrapings using a modified CTAB method previously described by Abarshi et al. (2010) and tested using specific primers for nine grapevine viruses by RT-PCR (Gambino & Gribaudo 2006). All samples tested positive for Grapevine rupestris stem pitting-associated virus (GRSPaV) and two of the samples, clones 119 and 792, had mixed infections with Grapevine fleck virus (GFkV) and Grapevine fanleaf virus (GFLV), respectively. 

PCR amplicons from GRSPaV and GFkV primer sets were sequenced in both directions, and similarity searches using BLAST in the GenBank databases showed that the GFkV sequence (GenBank Accession No. MF959546) has 97% nucleotide identity with isolate SK79 from Slovakia (JN133946), and GRSPaV sequences (MF959547 to MF959550) have 97 to 100% nucleotide identity with isolates from Italy (DQ278627, HE591388 and FN555301) and the UK (KP284454).

To confirm the detection of GFLV in the UK on Pinot Noir clone 792 a second RT-PCR was performed using GFLV-specific primers targeting the coat protein (CP) of the virus (Mekuria et al., 2008). An amplicon of the expected size (322 bp) was obtained and sequenced in both directions. BLAST searches revealed that the obtained sequence (MF576058) showed 92% nucleotide identity with the homologous CP region of GFLV isolates (DQ362924 and DQ362933) from Italy.

To our knowledge, this is the first report of GFLV in the UK. GFLV is responsible for fanleaf degeneration disease, one of the most damaging viral diseases of grapevine (Andret-Link et al., 2004) causing malformation of leaves and affecting fruit quality and yield. Immanuel et al. (2015a; 2015b) previously reported the presence of GFkV, GRSPaV, Grapevine leafroll-associated virus 1 and Grapevine virus A infecting grapevine in the UK. The presence of viruses from different genera (Ampelovirus, Foveavirus, Maculavirus, Nepovirus and Vitivirus) constitutes a threat to the UK's developing grapevine industry. Large scale surveys of commercial vineyards in the UK are needed to determine the presence and incidence of grapevine viruses and to evaluate their potential effects on vineyard productivity and fruit yields and quality.


References

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To cite this report: Silva G, Lecourt J, Clover GRG, Seal SE, 2017. First report of Grapevine fanleaf virus infecting grapevine in the United Kingdom. New Disease Reports 36, 9. [http://dx.doi.org/10.5197/j.2044-0588.2017.036.009]

©2017 The Authors